TNF alpha antibody (AA 80-227)

Catalog No. ABIN3043313

Product Details anti-TNF alpha Antibody

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Binding Specificity: AA 80-227

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-TNF alpha Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TNF alpha Antibody Picoband® (ABIN3043313). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse TNF alpha recombinant protein (Position: L80-Q227). Mouse TNF alpha shares 79% and 95% amino acid (aa) sequence identity with human and rat TNF alpha, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse
1. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B.Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985. 2. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V.Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TNF alpha antibody (ABIN3043313)

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Wang, Li, Chen, Jiang, Lu, Zhao: "Hydrogen sulfide accelerates wound healing in diabetic rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5097-104, (2016) (PubMed).

Pan, Wang, Pu, Yao, Wang: "Effect of Puerarin on Expression of ICAM-1 and TNF-α in Kidneys of Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 2134-40, (2016) (PubMed).

Chen, Wang, Li, Jiang, Lu, Zhou: "Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats." in: Journal of diabetes research, Vol. 2016, pp. 9726503, (2016) (PubMed).

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Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

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Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

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Chen, Zhao, Wu, Zou, Luo, Li, Xie, Liang: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).

Li, Zhang, Fu, Sun, Gong, Jiang, Zhang: "Dual pancreas- and lung-targeting therapy for local and systemic complications of acute pancreatitis mediated by a phenolic propanediamine moiety." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 212, pp. 19-29, (2015) (PubMed).

Li, Chen, Zhang, Song, Mu: "Gastrodin inhibits neuroinflammation in rotenone-induced Parkinson's disease model rats." in: Neural regeneration research, Vol. 7, Issue 5, pp. 325-31, (2015) (PubMed).

Li, Qiu, Wang, Niu, Pan: "Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury." in: Food & function, Vol. 6, Issue 2, pp. 470-8, (2015) (PubMed).

Xia, Li, Xiang, Chang, Xia, Han: "Neuroprotective effects of an aqueous extract of Futokadsura stem in an A?-induced Alzheimer's disease-like rat model." in: The Chinese journal of physiology, Vol. 58, Issue 2, pp. 104-13, (2015) (PubMed).

Xie, Zhang, Li, Xu: "Inhibition of inflammatory cytokines after early decompression may mediate recovery of neurological function in rats with spinal cord injury." in: Neural regeneration research, Vol. 10, Issue 2, pp. 219-24, (2015) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF (TNF alpha Products)

Background:

Synonyms: Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2;

Tissue Specificity: Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.

Background: TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Sequence Similarities: Belongs to the IL-15/IL-21 family.

Molecular Weight: 25 kDa

Gene ID: 21926

UniProt: P06804

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome