PLK1 antibody (AA 86-430)

Catalog No. ABIN3043354

This Rabbit Polyclonal antibody specifically detects PLK1 in WB. It exhibits reactivity toward Human.

Quick Overview for PLK1 antibody (AA 86-430) (ABIN3043354)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLK1 Antibody

Binding Specificity: AA 86-430

Purpose: Anti-PLK1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PLK1 Antibody Picoband® (ABIN3043354). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLK1 recombinant protein (Position: K86-N430). Human PLK1 shares 95.4% and 96.2% amino acid (aa) sequence identity with mouse and rat PLK1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Holtrich U, Wolf G, Bräuninger A, Karn T, Böhme B, Rübsamen-Waigmann H et al. (March 1994). "Induction and down-regulation of PLK, a human serine/threonine kinase expressed in proliferating cells and tumors". Proc. Natl. Acad. Sci. U.S.A. 91(5): 1736-40. 2. Soung NK, Park JE, Yu LR, Lee KH, Lee JM, Bang JK et al. (April 2009). "Plk1-dependent and -independent roles of an ODF2 splice variant, hCenexin1, at the centrosome of somatic cells". Dev. Cell 16(4): 539-50.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background:

Synonyms: Serine/threonine-protein kinase PLK1,2.7.11.21,Polo-like kinase 1,PLK-1,Serine/threonine-protein kinase 13,STPK13,PLK1,PLK,

Tissue Specificity: Placenta and colon.

Background: Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13 (STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.

Molecular Weight: 68 kDa

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase