TICAM1 antibody (N-Term)

Catalog No. ABIN3043539

Cited in 1 publication. The Rabbit Polyclonal anti-TICAM1 antibody (ABIN3043539) specifically detects TICAM1 in WB. The antibody is reactive with Mouse and Rat samples.

Quick Overview for TICAM1 antibody (N-Term) (ABIN3043539)

Target: TICAM1 (Toll-Like Receptor Adaptor Molecule 1 (TICAM1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TICAM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TICAM1 Antibody

Binding Specificity: N-Term

Purpose: Anti-TRIF/TICAM1 Antibody

Sequence: QDTEARVSLE SLKMNTVAQL VAHQWADMET TE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related human sequence by twelve amino acids.

Characteristics: Anti-TRIF/TICAM1 Antibody. Tested in WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse TRIF, different from the related human sequence by twelve amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-TICAM1 antibody (ABIN3043539)

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Target Details for TICAM1

Target: TICAM1 (Toll-Like Receptor Adaptor Molecule 1 (TICAM1))

Alternative Name: Ticam1

Background:

Background: TICAM1 (TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TRIF, is an adapter in responding to activation of toll-like receptors (TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al. (2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al. (2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. Small interfering (si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.

Gene Full Name: TIR domain containing adaptor molecule 1

Molecular Weight: 110 kDa

Gene ID: 106759

UniProt: Q80UF7

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades