FAK antibody (C-Term)

Catalog No. ABIN3043556

The Rabbit Polyclonal anti-FAK antibody has been validated for WB. It is suitable to detect FAK in samples from Human, Mouse and Rat. There are 2+ publications available.

Quick Overview for FAK antibody (C-Term) (ABIN3043556)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FAK Antibody

Binding Specificity: AA 1024-1052, C-Term

Purpose: Anti-FAK/PTK2 Antibody Picoband®

Sequence: AHALAVDAKN LLDVIDQARL KMLGQTRPH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FAK/PTK2 Antibody Picoband® (ABIN3043556). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PTK2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Beggs, H. E., Schahin-Reed, D., Zang, K., Goebbels, S., Nave, K.-A., Gorski, J., Jones, K. R., Sretavan, D., Reichardt, L. F.FAK deficiency in cells contributing to the basal lamina results in cortical abnormalities resembling congenital muscular dystrophies.Neuron 40: 501-514, 2003. 2. Li, W., Lee, J., Vikis, H. G., Lee, S.-H., Liu, G., Aurandt, J., Shen, T.-L., Fearon, E. R., Guan, J.-L., Han, M., Rao, Y., Hong, K., Guan, K.-L.Activation of FAK and Src are receptor-proximal events required for netrin signaling.Nature Neurosci. 7: 1213-1221, 2004.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-FAK antibody (ABIN3043556)

Su, Zhang, Mao, Liu, Zheng, Zhang, Su: "Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer." in: Oncology letters, Vol. 9, Issue 2, pp. 595-603, (2015) (PubMed).

Cao, Xing, Yang, Mei: "Correlation between matrix metalloproteinase expression and activation of the focal adhesion kinase signaling pathway in herpes stromal keratitis." in: Experimental and therapeutic medicine, Vol. 7, Issue 1, pp. 280-286, (2013) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: PTK2

Background:

Synonyms: Focal adhesion kinase 1,FADK 1,2.7.10.2,Focal adhesion kinase-related nonkinase,FRNK,Protein phosphatase 1 regulatory subunit 71,PPP1R71,Protein-tyrosine kinase 2,p125FAK,pp125FAK,PTK2,FAK, FAK1,

Tissue Specificity: Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. .

Background: PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Molecular Weight: 125 kDa

Gene ID: 5747

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling