Cytochrome C antibody (C-Term)

Catalog No. ABIN3043697

The Rabbit Polyclonal anti-Cytochrome C antibody has been validated for WB, IHC, FACS, IF, ICC and IHC (fro). It is suitable to detect Cytochrome C in samples from Human, Rat and Mouse. There are 19+ publications available.

Quick Overview for Cytochrome C antibody (C-Term) (ABIN3043697)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cytochrome C Antibody

Binding Specificity: AA 91-105, C-Term

Purpose: Anti-Cytochrome C/CYCS Antibody Picoband®

Sequence: ERADLIAYLK KATNE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cytochrome C/CYCS Antibody (ABIN3043697). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, Rat, Mouse
Immunocytochemistry , 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-μg/1x10⁶ cells, Human
1. Li, K., Li, Y., Shelton, J. M., Richardson, J. A., Spencer, E., Chen, Z. J., Wang, X., Williams, R. S. : Cytochrome c deficiency causes embryonic lethality and attenuates stress-induced apoptosis. Cell 101: 389-399, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Cytochrome C antibody (ABIN3043697)

Bai, Yang, Luo: "Effects of 5-hydroxy-4'-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells." in: Oncology letters, Vol. 15, Issue 4, pp. 5227-5235, (2018) (PubMed).

Jiao, Ma, Wang, Li, Shan, Sun, Chen: "Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress." in: International journal of biological sciences, Vol. 12, Issue 5, pp. 580-93, (2017) (PubMed).

Hu, Sun, Zhang, Zhang, Hu: "Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway." in: Bioscience reports, Vol. 36, Issue 3, (2017) (PubMed).

Gan, Liu, Cao, Zhang, Sun: "FABP4 reversed the regulation of leptin on mitochondrial fatty acid oxidation in mice adipocytes." in: Scientific reports, Vol. 5, pp. 13588, (2016) (PubMed).

Han, Liu, Li, Tian, Han, Wang, Fu, Guo, Zhu, Du, Tian: "HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142387, (2016) (PubMed).

Jiao, Ma, Wang, Li, Shan, Liu, Liu, Song, Yu, Yu, Liu, Huang, Chen: "N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147858, (2016) (PubMed).

Ji, Geng, Zhou, Wei, Chen: "Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells." in: Neural regeneration research, Vol. 11, Issue 12, pp. 1962-1968, (2016) (PubMed).

Xu, Liu, Zhang, Wang, Wang, Yang, Huo, Sun: "Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy." in: Neural regeneration research, Vol. 7, Issue 17, pp. 1318-24, (2015) (PubMed).

Ding, Wang, Zhao, Sun, Zhai: "Protective Effects of Baicalin on A?????-Induced Learning and Memory Deficit, Oxidative Stress, and Apoptosis in Rat." in: Cellular and molecular neurobiology, Vol. 35, Issue 5, pp. 623-32, (2015) (PubMed).

Li, Wang, Wang: "Radioprotective activity of neutral polysaccharides isolated from the fruiting bodies of Hohenbuehelia serotina." in: Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB), Vol. 31, Issue 4, pp. 352-9, (2015) (PubMed).

Huang, Lin, Su, Ye: "Forsythiaside protects against hydrogen peroxide-induced oxidative stress and apoptosis in PC12 cell." in: Neurochemical research, Vol. 40, Issue 1, pp. 27-35, (2015) (PubMed).

Zhang, Liu, Wang, Jin, Du, Peng, Xue: "Benzo(a)pyrene-7,8-diol-9,10-epoxide induced p53-independent necrosis via the mitochondria-associated pathway involving Bax and Bak activation." in: Human & experimental toxicology, Vol. 34, Issue 2, pp. 179-90, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Wei, Tian, Yan, Shao, Xie: "Protective effects of phillyrin on H2O 2-induced oxidative stress and apoptosis in PC12 cells." in: Cellular and molecular neurobiology, Vol. 34, Issue 8, pp. 1165-73, (2014) (PubMed).

Ban, Fan, Huang, Li, Chen, Zhang, Xu: "Mono-(2-ethylhexyl) phthalate induces injury in human umbilical vein endothelial cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e97607, (2014) (PubMed).

Lin, Tongyi: "Role of Bax/Bcl-2 family members in green tea polyphenol induced necroptosis of p53-deficient Hep3B cells." in: Tumour biology, Vol. 35, Issue 8, pp. 8065-75, (2014) (PubMed).

Mo, Chen, Huang, Zhang, Li, Zhou: "Neuroprotective effect of tea polyphenols on oxyhemoglobin induced subarachnoid hemorrhage in mice." in: Oxidative medicine and cellular longevity, Vol. 2013, pp. 743938, (2014) (PubMed).

Song, Yue, Li, Li, Zhao, Zhang: "Study of the mechanism of sonodynamic therapy in a rat glioma model." in: OncoTargets and therapy, Vol. 7, pp. 1801-10, (2014) (PubMed).

Li, Li, Huang, Kan, Wang, Wu, Yin, Yao: "Dexamethasone and A???-?? accelerate learning and memory impairments due to elevate amyloid precursor protein expression and neuronal apoptosis in 12-month male rats." in: Behavioural brain research, Vol. 227, Issue 1, pp. 142-9, (2011) (PubMed).

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS

Background:

Synonyms: Cytochrome c,CYCS,CYC,

Tissue Specificity: Widely expressed, with highest levels in heart, brain, and skeletal muscle. .

Background: Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system. Human cytochrome c has 104 amino acid residues and a molecular weight of 11,458 and is mapped to 7p15.2. Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. And it has a role in different apoptotic signaling cascades.

Sequence Similarities: Belongs to the cytochrome c family.

Molecular Weight: 14 kDa

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity