LCK antibody (C-Term)

Catalog No. ABIN3043870

This Rabbit Polyclonal antibody specifically detects LCK in WB, IHC, IF, FACS, ICC and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LCK antibody (C-Term) (ABIN3043870)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LCK Antibody

Binding Specificity: AA 468-506, C-Term

Purpose: Anti-Lck Antibody Picoband®

Sequence: ELYQLMRLCW KERPEDRPTF DYLRSVLEDF FTATEGQYQ

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Lck Antibody Picoband® (ABIN3043870). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Lck, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Anderson, S. J., Levin, S. D., Perlmutter, R. M. Protein tyrosine kinase p56(lck) controls allelic exclusion of T-cell receptor beta-chain genes. Nature 365: 552-554, 1993. 2. Burnett, R. C., David, J.-C., Harden, A. M., Le Beau, M. M., Rowley, J. D., Diaz, M. O. The LCK gene is involved in the t(1,7)(p34,q34) in the T-cell acute lymphoblastic leukemia derived cell line, HSB-2. Genes Chromosomes Cancer 3: 461-467, 1991. 3. Marth, J. D., Disteche, C., Pravtcheva, D., Ruddle, F., Krebs, E. G., Perlmutter, R. M. Localization of a lymphocyte-specific protein tyrosine kinase gene (lck) at a site of frequent chromosomal abnormalities in human lymphomas. Proc. Nat. Acad. Sci. 83: 7400-7404, 1986.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: LCK

Background:

Synonyms: Tyrosine-protein kinase Lck,2.7.10.2,Leukocyte C-terminal Src kinase,LSK,Lymphocyte cell-specific protein-tyrosine kinase,Protein YT16,Proto-oncogene Lck,T cell-specific protein-tyrosine kinase,p56-LCK,LCK,

Tissue Specificity: Expressed specifically in lymphoid cells.

Background: Lck (or lymphocyte-specific protein tyrosine kinase) is a 56 kDa protein that is found inside specializedcells of the immune system called lymphocytes. The human LCK gene is mapped to chromosome 1p35-p32. This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.

Molecular Weight: 58 kDa

Gene ID: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling