UPF3B antibody (C-Term)

Catalog No. ABIN3043956

Product Details anti-UPF3B Antibody

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Binding Specificity: AA 416-452, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UPF3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Regulator of nonsense transcripts 3B(UPF3B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: SEKTEKKEEV VKRDRIRNKD RPAMQLYQPG ARSRNRL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Regulator of nonsense transcripts 3B(UPF3B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: UPF3 regulator of nonsense transcripts homolog B (yeast)
Protein Name: Regulator of nonsense transcripts 3B

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human UPF3B /RENT3B (416-452aa SEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRL).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-UPF3B antibody (ABIN3043956)

Huang, Low, Damle, Keenan, Kuntz, Murray, Monia, Guo: "Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations." in: Genome biology, Vol. 19, Issue 1, pp. 4, (2018) (PubMed).

Target Details for UPF3B

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Alternative Name: UPF3B (UPF3B Products)

Synonyms: HUPF3B antibody, MRXS14 antibody, RENT3B antibody, UPF3X antibody, 5730594O13Rik antibody, AI317193 antibody, AW541158 antibody, RGD1560264 antibody, UPF3B, regulator of nonsense mediated mRNA decay antibody, UPF3 regulator of nonsense transcripts homolog B (yeast) antibody, UPF3B antibody, Upf3b antibody

Background: Regulator of nonsense transcripts 3B is a protein that in humans is encoded by the UPF3B gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

Synonyms: HUPF3B antibody|hUpf3p X antibody|MRXS14 antibody|Nonsense mRNA reducing factor 3B antibody|Regulator of nonsense transcripts 3B antibody|RENT3B antibody|Up-frameshift suppressor 3 homolog B antibody|Up-frameshift suppressor 3 homolog on chromosome X antibody| UPF3 regulator of nonsense transcripts homolog B (yeast) antibody|UPF3 regulator of nonsense transcripts homolog B antibody|UPF3B antibody|UPF3X antibody

Gene ID: 65109