SLC12A2 antibody (N-Term)

Catalog No. ABIN3043994

Product Details anti-SLC12A2 Antibody

Target: SLC12A2 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 2 (SLC12A2))

Binding Specificity: AA 223-241, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC12A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Solute carrier family 12 member 2(SLC12A2) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Sequence: RIDHYRHTAA QLGEKLLRP

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Solute carrier family 12 member 2(SLC12A2) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: solute carrier family 12(sodium/potassium/chloride transporters), member 2
Protein Name: Solute carrier family 12 member 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1(223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SLC12A2

Target: SLC12A2 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 2 (SLC12A2))

Alternative Name: SLC12A2 (SLC12A2 Products)

Synonyms: BSC antibody, BSC2 antibody, NKCC1 antibody, Bsc2 antibody, Nkcc1 antibody, bsc2 antibody, 9330166H04Rik antibody, mBSC2 antibody, sy-ns antibody, solute carrier family 12 member 2 antibody, solute carrier family 12, member 2 antibody, SLC12A2 antibody, Slc12a2 antibody

Background: Solute carrier family 12(sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.

Synonyms: Basolateral Na-K-Cl symporter antibody|BSC antibody|BSC2 antibody|Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody|mBSC2 antibody|MGC104233 antibody|NKCC1 antibody|S12A2_HUMAN antibody|SLC12A2 antibody|sodium-potassium-chloride cotransporter 1 antibody|solute carrier family 12(sodium/potassium/chloride transporters) member 2 antibody|Solute carrier family 12 member 2 antibody|sy-ns antibody

UniProt: P55011

Pathways: Sensory Perception of Sound