SLC12A2 antibody (Internal Region)

Catalog No. ABIN570824

The Goat Polyclonal anti-SLC12A2 antibody is suitable to detect SLC12A2 in samples from Human. It has been validated for ELISA, IHC, IF and FACS.

Quick Overview for SLC12A2 antibody (Internal Region) (ABIN570824)

Target: SLC12A2 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 2 (SLC12A2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC12A2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SLC12A2 Antibody

Binding Specificity: Internal Region

Purpose: SLC12A2

Sequence: NALQHSIRLS GVEDH

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NALQHSIRLSGVEDH, from the internal region of the protein sequence according to NP_001037.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SLC12A2

Target: SLC12A2 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 2 (SLC12A2))

Alternative Name: SLC12A2

Background: SLC12A2, solute carrier family 12 (sodium/potassium/chloride transporters), member 2, BSC, BSC2, MGC104233, NKCC1, basolateral Na-K-Cl symporter, bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1

Molecular Weight: 75kDa

Gene ID: 6558, 20496, 83629

NCBI Accession: NP_001037, NP_001243390

Pathways: Sensory Perception of Sound