MYBL2 antibody (N-Term)

Catalog No. ABIN3044010

Cited in 1 publication. The Rabbit Polyclonal anti-MYBL2 antibody (ABIN3044010) specifically detects MYBL2 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for MYBL2 antibody (N-Term) (ABIN3044010)

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYBL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MYBL2 Antibody

Binding Specificity: N-Term

Purpose: Anti-B MyB/MYBL2 Antibody

Sequence: MSRRTRCEDL DELHYQD

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse sequence,different from the related rat sequence by two amino acids.

Characteristics: Anti-B MyB/MYBL2 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human B MyB, identical to the related mouse sequence and different from the related rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-MYBL2 antibody (ABIN3044010)

Tao, Wang, Xu, Li, Li, Xu, Fang, Li, Qian, Li, Li, Wu, Ren, Du, Lu, Feng, Wang, He, Hu, Pan: "Molecular mechanism of G1 arrest and cellular senescence induced by LEE011, a novel CDK4/CDK6 inhibitor, in leukemia cells." in: Cancer cell international, Vol. 17, pp. 35, (2017) (PubMed).

Target Details for MYBL2

Target: MYBL2 (V-Myb Myeloblastosis Viral Oncogene Homolog (Avian)-Like 2 (MYBL2))

Alternative Name: MYBL2

Background:

Background: MYBL2 (V-MYB avian myeloblastosis viral oncogene homolog-like 2), also called MYB-RELATED GENE BMYB, is a protein that in humans is encoded by the MYBL2 gene. The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. Barletta et al. (1991) assigned the MYBL2 gene to chromosome Xq13. However, Noben-Trauth et al. (1996) demonstrated that this assignment was an error. Using mouse Mybl2 cDNA clones as probes, they assigned Mybl2 in an interspecific backcross panel to distal mouse chromosome 2. Using human cDNA probes in combination with fluorescence in situ hybridization analysis, they localized MYBL2 to chromosome 20q13.1, a region that is commonly deleted in myeloid disorders and shows high homology of synteny to mouse chromosome 2. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.

Gene Full Name: MYB proto-oncogene like 2

Sequence Similarities: Contains 3 HTH myb-type DNA-binding domains.

Molecular Weight: 100 kDa

Gene ID: 4605

UniProt: P10244

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Chromatin Binding