TRAF2 antibody (TNF Receptor-Associated Factor 2) (AA 305-325)

Details for Product anti-TRAF2 Antibody No. ABIN3044047, Supplier: Log in to see
Antigen
  • MGC:45012
  • TRAP
  • TRAP3
  • TANK
  • AI325259
  • TNF-receptor-associated factor 2
  • conjugal transfer protein TraF
  • TNF receptor associated factor 2
  • TNF receptor-associated factor 2
  • Tnf receptor-associated factor 2
  • LOC661716
  • traF2
  • TRAF2
  • Traf2
Alternatives
anti-Human TRAF2 antibody for Western Blotting
Epitope
AA 305-325, Middle Region
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Reactivity
Human, Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This TRAF2 antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Purpose Rabbit IgG polyclonal antibody for TNF receptor-associated factor 2(TRAF2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human TRAF2(305-325aa RQHRLDQDKIEALSSKVQQLE), different from the related rat and mouse sequences by one amino acid.
Sequence RQHRLDQDKI EALSSKVQQL E
Isotype IgG
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for TNF receptor-associated factor 2(TRAF2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: TNF receptor-associated factor 2
Protein Name: TNF receptor-associated factor 2
Purification Immunogen affinity purified.
Alternative Name TRAF2 (TRAF2 Antibody Abstract)
Background TRAF2(TNF Receptor-Associated Factor 2), also called TRAP, is a protein that in humans is encoded by the TRAF2 gene. The protein encoded by this gene is a member of the TNF receptor(TNFR) associated factor(TRAF) protein family. TRAF2 is a common signal transducer for TNFR2 and CD40 that mediates activation of NF-kappa-B. Rothe et al.(1996) identified ITRAF, which binds to TRAF1, TRAF2, and TRAF3, and that when overexpressed inhibits TRAF2-mediated NF-kappa-B activation. They proposed that ITRAF is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Kanamori et al.(2002) found that mouse Traf2 interacted directly with T2bp, and they presented evidence that T2BP is involved in TNF-mediated signaling by its interaction with TRAF2.

Synonyms: E3 ubiquitin-protein ligase TRAF2 antibody|MGC: 45012 antibody|OTTHUMP00000022625 antibody|OTTHUMP00000064745 antibody|TNF receptor associated factor 2 antibody|TNF receptor-associated factor 2 antibody|TNF receptor-associated protein antibody|TRAF 2 antibody|TRAF2 antibody|TRAF2_HUMAN antibody|TRAP 3 antibody|TRAP antibody|TRAP3 antibody|Tumor necrosis factor type 2 receptor associated protein 3 antibody|Tumor necrosis factor type 2 receptor-associated protein 3 antibody
UniProt Q12933
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded protein response
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for TRAF2 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-TNF Receptor-Associated Factor 2 (TRAF2) (AA 305-325), (Middle Region) antibody (ABIN3044047) Anti-TRAF2 antibody, ICC ICC: HELA Cell
Immunohistochemistry (IHC) image for anti-TNF Receptor-Associated Factor 2 (TRAF2) (AA 305-325), (Middle Region) antibody (ABIN3044047) Anti-TRAF2 antibody, IHC(P) IHC(P): Human Tonsil Tissue
Western Blotting (WB) image for anti-TNF Receptor-Associated Factor 2 (TRAF2) (AA 305-325), (Middle Region) antibody (ABIN3044047) anti-TNF Receptor-Associated Factor 2 (TRAF2) (AA 305-325), (Middle Region) antibody (Image 3)
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