ATM antibody (N-Term)

Catalog No. ABIN3044170

Product Details anti-ATM Antibody

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Binding Specificity: AA 36-50, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Serine-protein kinase ATM(ATM) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DPETIKHLDR HSDSK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Serine-protein kinase ATM(ATM) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: ataxia telangiectasia mutated
Protein Name: Serine-protein kinase ATM

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ATM(36-50aa DPETIKHLDRHSDSK), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM (ATM Products)

Synonyms: ATM antibody, Atm antibody, CG6535 antibody, Dmel\\CG6535 antibody, Tefu antibody, atm antibody, atm/tefu antibody, dATM antibody, tef antibody, Xatm antibody, at1 antibody, atdc antibody, tel1 antibody, telo1 antibody, AT1 antibody, ATA antibody, ATC antibody, ATD antibody, ATDC antibody, ATE antibody, TEL1 antibody, TELO1 antibody, AI256621 antibody, C030026E19Rik antibody, telomere fusion antibody, ATM serine/threonine kinase L homeolog antibody, ATM serine/threonine kinase antibody, ataxia telangiectasia mutated antibody, ataxia telangiectasia mutated (atm) antibody, serine/threonine-protein kinase ATM antibody, tefu antibody, atm.L antibody, atm antibody, ATM antibody, EDI_100660 antibody, CpipJ_CPIJ001772 antibody, BDBG_08252 antibody, PAAG_02532 antibody, MCYG_05088 antibody, VDBG_06833 antibody, ACLA_015700 antibody, LOC5565620 antibody, MGYG_07634 antibody, PGTG_14279 antibody, Atm antibody

Background: ATM(ataxia telangiectasia mutated),also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. Linkage analysis of ataxia-telangiectasia led to mapping of the ATM gene to chromosome 11q22.3. Using an antiserum developed to a peptide corresponding to the deduced amino acid sequence of ATM, the ATM protein is a single, high molecular weight protein predominantly confined to the nucleus of human fibroblasts, although it is present in both nuclear and microsomal fractions from human lymphoblast cells and peripheral blood lymphocytes. Overexpression of ATM cDNA in AT cells enhanced their survival after radiation exposure, decreased radiation-induced chromosome aberrations, reduced radioresistant DNA synthesis, and partially corrected defective cell cycle checkpoints and induction of stress-activated protein kinase. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors, in a telomere-independent manner. ATM functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.

Synonyms: A-T mutated antibody|A-T mutated homolog antibody|AT complementation group A antibody|AT complementation group C antibody|AT complementation group D antibody|AT complementation group E antibody|AT mutated antibody|AT protein antibody|AT1 antibody|ATA antibody|Ataxia telangiectasia gene mutated in human beings antibody|Ataxia telangiectasia mutated antibody|Ataxia telangiectasia mutated gene antibody|Ataxia telangiectasia mutated homolog(human) antibody|Ataxia telangiectasia mutated homolog antibody|ATC antibody|ATD antibody|ATDC antibody|ATE antibody|ATM antibody|ATM_HUMAN antibody|DKFZp781A0353 antibody|Human phosphatidylinositol 3 kinase homolog antibody|MGC74674 antibody|OTTHUMP00000232981 antibody|Serine protein kinase ATM antibody|Serine-protein kinase ATM antibody|Serine/threonine-protein kinase ATM antibody|T cell prolymphocytic leukemia antibody|Tefu antibody|TEL1 antibody|TEL1, telomere maintenance 1, homolog antibody|TELO1 antibody|Telomere fusion protein antibody|TPLL antibody

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus