CISH antibody (C-Term)

Catalog No. ABIN3044172

The Rabbit Polyclonal anti-CISH antibody (ABIN3044172) specifically detects CISH in WB, IHC and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CISH antibody (C-Term) (ABIN3044172)

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CISH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CISH Antibody

Binding Specificity: C-Term

Purpose: Anti-CISH Antibody

Sequence: LPLPRRMADY LRQYPFQL

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-CISH Antibody. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CISH, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, MouseWestern blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CISH

Target: CISH (Cytokine Inducible SH2-Containing Protein (CISH))

Alternative Name: CISH

Background:

Background: CISH (cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling (SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide (LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.

Gene Full Name: cytokine inducible SH2 containing protein

Sequence Similarities: Contains 1 SH2 domain.

Molecular Weight: 29 kDa

Gene ID: 1154

UniProt: Q9NSE2

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus