HLA-DMB antibody (Middle Region)

Catalog No. ABIN3044175

Product Details anti-HLA-DMB Antibody

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Binding Specificity: AA 81-100, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for HLA class II histocompatibility antigen, DM beta chain(HLA-DMB) detection. Tested with WB, IHC-P in Human.

Sequence: NQKDTLMQRL RNGLQNCATH

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for HLA class II histocompatibility antigen, DM beta chain(HLA-DMB) detection. Tested with WB, IHC-P in Human.
Gene Name: major histocompatibility complex, class II, DM beta
Protein Name: HLA class II histocompatibility antigen, DM beta chain

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HLA DMB(81-100aa NQKDTLMQRLRNGLQNCATH).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HLA-DMB

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Alternative Name: HLA-DMB (HLA-DMB Products)

Synonyms: RING7 antibody, D6S221E antibody, RT1.DMb antibody, RT1.Mb antibody, MHC-DMB antibody, major histocompatibility complex, class II, DM beta antibody, RT1 class II, locus DMb antibody, HLA-DMB antibody, RT1-DMb antibody, PATR-DMB antibody

Background: HLA-DMB(major histocompatibility complex, class II, DM beta), also known as D6S221E, RING7, HLA-DM histocompatibility type, beta chain, HLADMB or RING7, is a protein that in humans is encoded by the HLA-DMB gene. The HLA-DMB gene is mapped on 6p21.32. HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha(DMA) and a beta(DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP(class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. HLA-DMA and -DMB appear to encode subunits of a functional heterodimer that is critical in the pathway of class II antigen presentation.

Synonyms: class II antigen HLA-DM beta chain antibody|class II histocompatibility antigen antibody|Class II histocompatibility antigen M beta chain antibody|D6S221E antibody|DMB antibody|HLA class II histocompatibility antigen DM beta chain antibody|HLA-DM histocompatibility type, beta chain antibody|M beta chain antibody|Major histocompatibility complex class II DM beta antibody|MHC class II antigen DMB antibody|MHC class II antigen HLA DM beta chain antibody|MHC class II HLA DMB antibody|OTTHUMP00000029257 antibody|Really interesting new gene 7 protein antibody|RING 7 antibody|RING7 antibody

UniProt: P28068

Pathways: Cancer Immune Checkpoints