HLA-DMB antibody (Middle Region)

Catalog No. ABIN3044175

The Rabbit Polyclonal anti-HLA-DMB antibody (ABIN3044175) specifically detects HLA-DMB in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for HLA-DMB antibody (Middle Region) (ABIN3044175)

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HLA-DMB Antibody

Binding Specificity: AA 81-100, Middle Region

Purpose: Anti-HLA DMB/HLA-DMB Antibody

Sequence: NQKDTLMQRL RNGLQNCATH

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HLA DMB/HLA-DMB Antibody (ABIN3044175). Tested in IHC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HLA DMB.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HLA-DMB

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Alternative Name: HLA-DMB

Background:

Synonyms: HLA class II histocompatibility antigen, DM beta chain,MHC class II antigen DMB,Really interesting new gene 7 protein,HLA-DMB,DMB, RING7,

Background: HLA-DMB (major histocompatibility complex, class II, DM beta), also known as D6S221E, RING7, HLA-DM histocompatibility type, beta chain, HLADMB or RING7, is a protein that in humans is encoded by the HLA-DMB gene. The HLA-DMB gene is mapped on 6p21.32. HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. HLA-DMA and -DMB appear to encode subunits of a functional heterodimer that is critical in the pathway of class II antigen presentation.

Sequence Similarities: Belongs to the MHC class II family.

Molecular Weight: 29 kDa

UniProt: P28068

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response