Apoptosis Inhibitor 5 antibody (C-Term)

Catalog No. ABIN3044316

The Rabbit Polyclonal anti-Apoptosis Inhibitor 5 antibody (ABIN3044316) specifically detects Apoptosis Inhibitor 5 in WB, IHC, IF, ICC and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Apoptosis Inhibitor 5 antibody (C-Term) (ABIN3044316)

Target: Apoptosis Inhibitor 5 (API5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apoptosis Inhibitor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Apoptosis Inhibitor 5 Antibody

Binding Specificity: C-Term

Purpose: Anti-Apoptosis inhibitor 5/API5 Antibody

Sequence: KYSSNLGNFN YERSLQGK

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse sequence by one amino acid.

Characteristics: Anti-Apoptosis inhibitor 5/API5 Antibody. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Apoptosis Inhibitor 5

Target: Apoptosis Inhibitor 5 (API5)

Alternative Name: API5

Background:

Background: Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death (apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Gene Full Name: apoptosis inhibitor 5

Sequence Similarities: Belongs to the API5 family.

Molecular Weight: 59 kDa

Gene ID: 8539

UniProt: Q9BZZ5

Pathways: Growth Factor Binding