GRIN1/NMDAR1 antibody (N-Term)

Catalog No. ABIN3044347

This Rabbit Polyclonal antibody specifically detects GRIN1/NMDAR1 in WB and IHC. It exhibits reactivity toward Human, Rat and Mouse. It has been mentioned in 3+ publications

Quick Overview for GRIN1/NMDAR1 antibody (N-Term) (ABIN3044347)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GRIN1/NMDAR1 Antibody

Binding Specificity: N-Term

Purpose: Anti-NMDAR1/GRIN1 Antibody

Sequence: RKHEQMFREA VNQANKRH

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related rat,mouse sequences.

Characteristics: Anti-NMDAR1/GRIN1 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-GRIN1/NMDAR1 antibody (ABIN3044347)

Feng, Du: "Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injury-induced coma: orexin-A-mediated N-methyl-D-aspartate receptor subunit NR1 upregulation." in: Neural regeneration research, Vol. 11, Issue 6, pp. 951-6, (2016) (PubMed).

Wang, Wang, Fu, Zhang, Wu, Guo, Wang: "Expression of N-methyl-d-aspartate receptor 1 and its phosphorylated state in basal ganglia of a neonatal piglet hypoxic-ischemic brain injury model: a controlled study of (1)H MRS." in: European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society, Vol. 16, Issue 5, pp. 492-500, (2012) (PubMed).

Shang, Luo, Deng, Wang, Huang, Feng, Yue: "Expression of N-methyl-D-aspartate receptor and its effect on nitric oxide production of rat alveolar macrophages." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, Vol. 23, Issue 4, pp. 327-31, (2010) (PubMed).

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GRIN1

Background:

Background: Glutamate [NMDA] receptor subunit zeta-1 is a protein that in humans is encoded by the GRIN1 gene. The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.

Gene Full Name: glutamate ionotropic receptor NMDA type subunit 1

Sequence Similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.

Molecular Weight: 110 kDa

Gene ID: 2902

UniProt: Q05586

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity