IGF1 antibody (Middle Region)

Catalog No. ABIN3044390

The Rabbit Polyclonal anti-IGF1 antibody has been validated for WB and IHC. It is suitable to detect IGF1 in samples from Human, Mouse and Rat. There are 6+ publications available.

Quick Overview for IGF1 antibody (Middle Region) (ABIN3044390)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IGF1 Antibody

Binding Specificity: AA 80-96, Middle Region

Purpose: Anti-Insulin-like growth factor I IGF1 Antibody Picoband®

Sequence: GSSSRRAPQT GIVDECC

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Insulin-like growth factor I IGF1 Antibody (ABIN3044390). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human IGF-1, different from the mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human
1. Höppener JW, de Pagter-Holthuizen P, Geurts van Kessel AH, Jansen M, Kittur SD, Antonarakis SE, Lips CJ, Sussenbach JS (1985). "The human gene encoding insulin-like growth factor I is located on chromosome 12". Hum. Genet. 69 (2): 157-60. 2. Jansen M, van Schaik FM, Ricker AT, Bullock B, Woods DE, Gabbay KH, Nussbaum AL,Sussenbach JS, Van den Brande JL (1983). "Sequence of cDNA encoding human insulin-like growth factor I precursor". Nature 306 (5943): 609-11.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-IGF1 antibody (ABIN3044390)

Wan, Wang, Xiong, Xiang, Li, Wang, Liu, Niu, Peng, Jiang, Chai: "Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing." in: Scientific reports, Vol. 6, pp. 36589, (2018) (PubMed).

Li, Zhang, Li, Zhao, Zhai, Yang, Kong, Wu, Chen, Teng: "miR-18a counteracts AKT and ERK activation to inhibit the proliferation of pancreatic progenitor cells." in: Scientific reports, Vol. 7, pp. 45002, (2017) (PubMed).

Huang, Zhou, Shi, Shao, Wang, Wang, Wang, Ding: "Effects of the IGF-1/PTEN/Akt/FoxO signaling pathway on male reproduction in rats subjected to water immersion and restraint stress." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5116-5124, (2017) (PubMed).

Tang, Liao, Xu, Zhang, Liu, Deng, Jiang, Fu, Chen, Zhou: "Estrogen and insulin-like growth factor 1 synergistically promote the development of lung adenocarcinoma in mice." in: International journal of cancer. Journal international du cancer, Vol. 133, Issue 10, pp. 2473-82, (2013) (PubMed).

Huang, Zhou, Wang, Wei, Zhang, Zhou, Hutz, Shi: "Effect of the IGF-1/PTEN/Akt/FoxO signaling pathway on the development and healing of water immersion and restraint stress-induced gastric ulcers in rats." in: International journal of molecular medicine, Vol. 30, Issue 3, pp. 650-8, (2012) (PubMed).

Chai, Guo, Wang, Fu, Guan, Tan, Ren, Yang: "Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo." in: Journal of materials science. Materials in medicine, Vol. 22, Issue 11, pp. 2525-35, (2011) (PubMed).

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1

Background:

Synonyms: Insulin-like growth factor I,IGF-I,Mechano growth factor,MGF,Somatomedin-C,IGF1,IBP1,

Tissue Specificity: Detected in brain cortex (at protein level). Detected in blood platelets. .

Background: Insulin-like growth factor 1 (IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al. (1990) placed the mouse IGF1 gene on chromosome 10.

Sequence Similarities: Belongs to the insulin family.

Molecular Weight: 50 kDa

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation