FGF19 antibody (Middle Region)

Catalog No. ABIN3044409

This anti-FGF19 antibody is a Rabbit Polyclonal antibody detecting FGF19 in WB and IHC. Suitable for Human.

Quick Overview for FGF19 antibody (Middle Region) (ABIN3044409)

Target: FGF19 (Fibroblast Growth Factor 19 (FGF19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FGF19 Antibody

Binding Specificity: AA 124-140, Middle Region

Purpose: Anti-Fibroblast growth factor 19 FGF19 Antibody Picoband®

Sequence: EEIRPDGYNV YRSEKHR

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Fibroblast growth factor 19 FGF19 Antibody (ABIN3044409). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human FGF19.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Kir, S., Beddow, S. A., Samuel, V. T., Miller, P., Previs, S. F., Suino-Powell, K., Xu, H. E., Shulman, G. I., Kliewer, S. A. , Mangelsdorf, D. J.FGF19 as a postprandial, insulin-independent activator of hepatic protein and glycogen synthesis. 2. Ladher, R. K., Anakwe, K. U., Gurney, A. L., Schoenwolf, G. C., Francis-West, P. H.Identification of synergistic signals initiating inner ear development. 3. Nishimura, T., Utsunomiya, Y., Hoshikawa, M., Ohuchi, H., Itoh, N.Structure and expression of a novel human FGF, FGF-19, expressed in the fetal brain. Biochim. Biophys. Acta 1444: 148-151, 1999.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FGF19

Target: FGF19 (Fibroblast Growth Factor 19 (FGF19))

Alternative Name: FGF19

Background:

Synonyms: Fibroblast growth factor 19,FGF-19,FGF19,UNQ334/PRO533,

Tissue Specificity: Expressed in fetal brain, cartilage, retina, and adult gall bladder. .

Background: FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.

Molecular Weight: 24 kDa

UniProt: O95750

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway