BCAT1 antibody (C-Term)

Catalog No. ABIN3044434

Product Details anti-BCAT1 Antibody

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Binding Specificity: AA 360-375, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) detection. Tested with WB, IHC-P in Human.

Sequence: KLASRILSKL TDIQYGR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) detection. Tested with WB, IHC-P in Human.
Gene Name: branched chain amino-acid transaminase 1, cytosolic
Protein Name: Branched-chain-amino-acid aminotransferase, cytosolic(BCAT(c))

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1(360-375aa KLASRILSKLTDIQYGR), different from the related mouse sequence by one amino acid, rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BCAT1

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Alternative Name: BCAT1 (BCAT1 Products)

Synonyms: fj66g02 antibody, zgc:73157 antibody, wu:fj66g02 antibody, Bcatc antibody, BCATC antibody, BCT1 antibody, ECA39 antibody, MECA39 antibody, PNAS121 antibody, PP18 antibody, BCATc antibody, Eca39 antibody, branched chain amino-acid transaminase 1, cytosolic antibody, branched chain amino acid transaminase 1 antibody, branched chain amino-acid transaminase 1, cytosolic L homeolog antibody, branched chain aminotransferase 1, cytosolic antibody, bcat1 antibody, BCAT1 antibody, bcat1.L antibody, Bcat1 antibody

Background: BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

Synonyms: BCAT antibody|BCAT(c) antibody|Bcat1 antibody|BCAT1_HUMAN antibody|BCT1 antibody|Branched chain amino acid aminotransferase, cytosolic antibody|branched chain amino-acid transaminase 1, cytosolic antibody|branched chain aminotransferase 1 antibody|branched chain aminotransferase 1, cytosolic antibody|Branched-chain-amino-acid aminotransferase antibody|cytosolic antibody|DKFZp686E12175 antibody|EC 2.6.1.42 antibody|ECA39 antibody|MECA39 antibody|placental protein 18 antibody|PNAS 121 antibody|PP18 antibody|Protein ECA39 antibody

UniProt: P54687