JUP antibody (AA 732-745)

Catalog No. ABIN305904

The Goat Polyclonal anti-JUP antibody (ABIN305904) specifically detects JUP in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Mouse, Dog, Rabbit, Monkey and Hamster samples.

Quick Overview for JUP antibody (AA 732-745) (ABIN305904)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human, Mouse, Dog, Rabbit, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JUP Antibody

Binding Specificity: AA 732-745

Specificity: Human JUP / Gamma Catenin. This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Dog, Hamster, Panda, Rabbit (100%) Rat, Bat, Bovine, Pig, Opossum (93%) Elephant (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-GLRPPYPTADHMLA from the C-terminus of human JUP / Gamma Catenin (NP_002221.1, NP_068831.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Dog, Hamster, Panda, Rabbit (100%), Rat, Bat, Bovine, Pig, Opossum (93%), Elephant (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP/CTNNG/Junction Plakoglobin

Background: Name/Gene ID: JUP
Family: Catenin

Synonyms: JUP, Desmoplakin-3, CTNNG, DPIII, Gamma catenin, Junction plakoglobin, PKGB, ARVD12, Catenin gamma, Desmoplakin III, DP3, PDGB

Gene ID: 3728

NCBI Accession: NP_002221, NP_068831

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location