LEF1 antibody (full length)

Catalog No. ABIN306681

The Mouse Monoclonal anti-LEF1 antibody (Clone REMB6) (ABIN306681) specifically detects LEF1 in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for LEF1 antibody (full length) (ABIN306681)

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: REMB6

Product Details anti-LEF1 Antibody

Binding Specificity: full length

Purpose: Mouse anti Human LEF-1

Characteristics: The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDSPAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB6 recognizes the High Mobility Group DNA domain of LEF-1

Purification: Protein A/G Chromatography

Immunogen: Full-length recombinant LEF-1 protein

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1-5 μg/mL) and immunofluorescence on paraformaldehyde-fixed cells (1:1000 or 1 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF/TCF/HMG

Background: Lef-1, mouse anti human lymphoid enhancer factor-1, T-cell specific transcription factor 1-alpha, TCF-1alpha

UniProt: Q9UJU2

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding