BAX antibody

Catalog No. ABIN306834

This anti-BAX antibody is a Sheep Polyclonal antibody detecting BAX in WB and IP. Suitable for Mouse.

Quick Overview for BAX antibody (ABIN306834)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-BAX Antibody

Cross-Reactivity: Mouse

Characteristics: Apoptosis regulator Bax cytoplasmic isoform beta , BaxB, BAX, BCL2 associated X protein, Apoptosis regulator BAX, BCL2 associated X protein isoform beta, Bax beta, Bax B,Bax is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax protein before a stop codon is encountered during translation. The Bax protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain (Oltvai et al., 1993). Bax mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax mRNA was very abundant in brain and duodenum with almost no Bax mRNA detected in these tissues (Oltvai et al., 1993).

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide corresponding to amino acids 172 to 199 of theBax protein

Isotype: IgG

Application Details

Application Notes: Antibody detects Bax? protein by immunoprecipitation only. Will not detect denatured protein by Western blot. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax-beta

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response