p53 antibody (full length)

Catalog No. ABIN306868

This Mouse Monoclonal antibody specifically detects p53 in WB, IHC (p) and IP. It exhibits reactivity toward Human.

Quick Overview for p53 antibody (full length) (ABIN306868)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: BP53-12

Product Details anti-p53 Antibody

Binding Specificity: AA 20-25, full length

Purpose: Mouse anti Human only p53 (a.a. 20-25)

Cross-Reactivity: Human

Characteristics: p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from immunized with full length wild-type human p53 protein and mouse myeloma cells.

Isotype: IgG2a

Application Details

Application Notes: This antibody can be used for Western blotting (0.5-1 μg/mL), immunohistochemistry on formalin/paraffain embedded tissues (1-2 μg/mL) and immunoprecipitation (2 μg/mg of protein lysate). Antibody reacts with mutant as well as wild-type p53 protein. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect