SPHK1 antibody

Catalog No. ABIN306932

The Rabbit Polyclonal anti-SPHK1 antibody is suitable to detect SPHK1 in samples from Human, Mouse, Rat and Cow. It has been validated for WB and ELISA.

Quick Overview for SPHK1 antibody (ABIN306932)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SPHK1 Antibody

Purpose: Rabbit anti Human Sphingosine Kinase 1

Cross-Reactivity: Cow, Human, Mouse, Rat

Characteristics: Sphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).

Purification: Antigen Immunoaffiinity Purification

Isotype: IgG

Application Details

Application Notes: Antibody can be used for Western blotting (1:400 dilution) and ELISA. Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: Sphingosine Kinase 1

Background: EC 2.7.1, SK 1, SPK 1, SPHK1, Gene name: SPHK1 or SPHK or SP

UniProt: Q9NYA1

Pathways: VEGF Signaling