EBF1 antibody (Internal Region)

Catalog No. ABIN308389

This Goat Polyclonal antibody specifically detects EBF1 in WB, ELISA and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for EBF1 antibody (Internal Region) (ABIN308389)

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This EBF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-EBF1 Antibody

Binding Specificity: Internal Region

Purpose: EBF1 / COE1

Sequence: HNQLPALANT SV

Specificity: This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HNQLPALANTSV, from the internal region of the protein sequence according to NP_076870.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 75 kDa band observed in Human Adipose lysates (calculated MW of 64.5 kDa according to NP_076870.1). Recommended concentration: 0.3-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EBF1

Target: EBF1 (Early B-Cell Factor 1 (EBF1))

Alternative Name: EBF1

Background: EBF1, early B-cell factor 1 , COE1, EBF, O/E-1, OLF1 , Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1

Gene ID: 1879, 13591, 116543

NCBI Accession: NP_076870