CSF1R antibody (PE)

Catalog No. ABIN317385

The PE-conjugated Rat Monoclonal anti-CSF1R antibody (Clone 604B5 2E11) (ABIN317385) specifically detects CSF1R in FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for CSF1R antibody (PE) (ABIN317385)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This CSF1R antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 604B5 2E11

Product Details anti-CSF1R Antibody (PE)

Specificity: This antibody recognizes the CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.

Cross-Reactivity (Details): Species reactivity (tested):Mouse and Rat.

Characteristics: Synonyms: CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1receptor

Purification: Purified IgG prepared by ion exchange chromatography

Immunogen: RAW 264 cells. Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CSF1R

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CD115

Background: CD115, also known as c-fms, macrophage Colony Stimulating Factor Receptor (M-CSFR), or CSF-1R, exists in immature (gp130c-fms) and mature (gp150c-fms) forms. CSF-1R exhibits an intrinsic tyrosine-specific protein kinase activity. It is expressed on the cells of the mononuclear phagocyte lineage. CSF-1R is an integral transmembrane glycoprotein consisting of a 512 amino acid extracellular domain that includes the CSF-1 binding site, a single 25 amino acid membrane-spanning segment, and a 435 amino acid cytoplasmic portion that includes the tyrosine kinase domain. CD115 is expressed by cells of the monocytic lineage and by progenitor cells.Synonyms: CSF-1-R, CSF1R, FMS, Fms proto-oncogene, Macrophage colony-stimulating factor 1 receptor, c-fms

Gene ID: 12978

UniProt: P09581

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization