Metabotropic Glutamate Receptor 5 antibody

Catalog No. ABIN317705

The Rabbit Polyclonal anti-Metabotropic Glutamate Receptor 5 antibody is suitable to detect Metabotropic Glutamate Receptor 5 in samples from Rat, Human and Mouse. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for Metabotropic Glutamate Receptor 5 antibody (ABIN317705)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Specificity: This antibody detects endogenous levels of mGluR5 / GRM5 protein (region surrounding Lys56).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-chromatography

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: mGluR5 / GRM5

Background: The mGluR proteins (metabotropic glutamate receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic glutamate receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR-1 and mGluR-5 are mediated by a G protein that activates a phosphatidylinositolcalcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight subtypes of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-3, which may interact with GRASP, acts as a receptor for glutamate.Synonyms: GPRC1E, MGLUR5, Metabotropic glutamate receptor 5

Molecular Weight: approx. 132 kDa

Gene ID: 2915

NCBI Accession: NP_000833

UniProt: P41594

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity