FFAR1 antibody

Catalog No. ABIN317749

This Rabbit Polyclonal antibody specifically detects FFAR1 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FFAR1 antibody (ABIN317749)

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-FFAR1 Antibody

Specificity: This antibody detects endogenous levels of GPR40 protein. (region surrounding Trp224)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FFAR1

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Alternative Name: FFAR1 / GPR40

Background: G protein coupled receptors provide attractive targets for drug therapy due to the sheer size and diversity of ligands within this receptor family. G proteincoupled receptor 40 (GPR40) functions as a cell-surface receptor for longchain free fatty acids (FFAs). FFAs provide an important energy source, but also function as signaling molecules in various pathways, notably the process of insulin secretion. In pancreatic tissue, the interaction of long chain FFAs with GPR40 amplifies glucose-stimulated insulin secretion from beta cells, suggesting a possible role for GPR40 in the treatment of diabetes associated with insulin-deficiency. Specifically, the Arg211His polymorphism in the GPR40 gene may contribute to the variation of insulin secretory capacity in Japanese men. Also, GPR40 may be involved in the control of breast cancer cell growth by fatty acids and, therefore, provide a link between fat and cancer.Synonyms: Free fatty acid receptor 1, G-protein coupled receptor 40

Molecular Weight: approx. 26 kDa

Gene ID: 2864

NCBI Accession: NP_005294

UniProt: O14842

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis