p53 antibody (2meLys370)

Catalog No. ABIN3181859

The Rabbit Polyclonal anti-p53 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect p53 in samples from Human.

Quick Overview for p53 antibody (2meLys370) (ABIN3181859)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-p53 Antibody

Binding Specificity: 2meLys370

Specificity: Di-Methyl-p53 (K370) Polyclonal Antibody detects endogenous levels of p53 protein only when di-methylated at K370.

Characteristics: Rabbit Polyclonal to Di-Methyl-p53 (K370).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human p53 around the di-methylation site of K370.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC-P 1:100-300, ELISA 1:20000,

Comment:

CV: also recognized unmethylate p53

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Molecular Weight: 53 kDa

Gene ID: 7157

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect