Collagen Type I antibody

Catalog No. ABIN318505

This Rabbit Polyclonal antibody specifically detects Collagen Type I in IHC (fro), IF, IHC (p), RIA and EIA. It exhibits reactivity toward Mouse.

Quick Overview for Collagen Type I antibody (ABIN318505)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Specificity: This antibody is specific for Mouse Collagen type I. Less than 1 % cross reactivity has been observed with Mouse Collagen, types II, VI and III and Human, Chicken and Rat Collagen, type I.

Purification: Purified Ig prepared by affinity chromatography

Immunogen: Native type I Collagen from Mouse skin.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/100-1/400. Western Blot. Immunofluorescence: 1/50-1/100. Radioimmunoassays. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/250-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate buffered saline containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 12842

NCBI Accession: NP_031768

UniProt: P11087