Collagen Type I antibody

Catalog No. ABIN318518

The Rabbit Polyclonal anti-Collagen Type I antibody has been validated for IHC (fro), IF and EIA. It is suitable to detect Collagen Type I in samples from Human and Pig.

Quick Overview for Collagen Type I antibody (ABIN318518)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-Collagen Type I Antibody

Specificity: This antibody reacts with native and heat denatured Porcine Collagen type 1 and 3. Less than 0.5 % reactivity is observed with Porcine Albumin and Immunoglobulins.

Cross-Reactivity (Details): Also reacts to: type III

Purification: Purified

Immunogen: Porcine Collagen, types 1 and 3 from skin

Isotype: IgG

Application Details

Application Notes: Immunofluorescence: 1/10-1/40. ELISA: 1/500-1/2000. Immunohistochemistry on Frozen Sections: 1/10-1/40.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore the vial (50 μg) with 500 μL of sterile distilled water or saline.

Buffer: Mannitol, dextran and salts.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I

Background: Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations, these mutations alter splicing, often leading to the exclusion of multiple exons.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452