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Recombinant 5-Hydroxymethylcytosine antibody

This Rabbit Monoclonal antibody specifically detects 5-Hydroxymethylcytosine in ELISA, DB, ICC, MeDIP, IHC and FACS. It exhibits reactivity toward Vertebrate.
Catalog No. ABIN3199278

Quick Overview for Recombinant 5-Hydroxymethylcytosine antibody (ABIN3199278)

Target

5-Hydroxymethylcytosine (5-hmC)

Antibody Type

Recombinant Antibody

Reactivity

  • 3
  • 1
Vertebrate

Host

  • 11
  • 5
Rabbit

Clonality

  • 10
  • 6
Monoclonal

Conjugate

  • 11
  • 1
  • 1
  • 1
  • 1
  • 1
This 5-Hydroxymethylcytosine antibody is un-conjugated

Application

  • 9
  • 9
  • 4
  • 4
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Dot Blot (DB), Immunocytochemistry (ICC), Methylated DNA Immunoprecipitation (MeDIP), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone

RM236
  • Purpose

    Rabbit monoclonal to 5-hydroxymethylcytosine (5-hmC), 5'-hmC

    Specificity

    This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

    Purification

    Protein A affinity purified from an animal origin-free culture supernatant

    Immunogen

    BSA-conjugated 5-hydroxymethylcytosine.

    Isotype

    IgG
  • Application Notes

    Dot Blot: 0.2 μg/mL - 1 μg/mL, ELISA: 0.1 μg/mL - 1 μg/mL, IHC: 0.1 μg/mL - 1 μg/mL, ICC: 0.5 μg/mL- 2 μg/mL, hMeDIP: 0.2 μg/mL- 2 μg/mL, FC: 1 μg/mL - 5 μg/mL

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    50 % Glycerol/PBS with 1 % BSA and 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    -20.0°C
  • Target

    5-Hydroxymethylcytosine (5-hmC)

    Target Type

    Chemical
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