E2F1 antibody (pSer364)

Catalog No. ABIN3201010

This Mouse Monoclonal antibody specifically detects E2F1 in WB and ELISA. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for E2F1 antibody (pSer364) (ABIN3201010)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: #2

Product Details anti-E2F1 Antibody

Binding Specificity: pSer364

Characteristics: Product: Mouse monoclonal antibody (clone #2) specific to the human E2F1 protein phosphorylated at Ser364. Produced in serum-free medium and purified under mild conditions

Purification: Purified

Immunogen: A synthetic peptide corresponding to a sequence of human E2F1 protein including and surrounding pSer364

Isotype: IgG2b kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL
2. ELISA
Not tested for other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (-), 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-E2F1 antibody (ABIN3201010)

Trimarchi, Lees: "Sibling rivalry in the E2F family." in: Nature reviews. Molecular cell biology, Vol. 3, Issue 1, pp. 11-20, (2002) (PubMed).

Irwin, Marin, Phillips, Seelan, Smith, Liu, Flores, Tsai, Jacks, Vousden, Kaelin: "Role for the p53 homologue p73 in E2F-1-induced apoptosis." in: Nature, Vol. 407, Issue 6804, pp. 645-8, (2000) (PubMed).

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: E2F1 is a member of E2F group of proteins that share common structural and functional domains and plays a major role during the G1/S transition in the mammalian cell cycle as a transcriptional factor. E2F1 is regulated during cell cycle progression. It is phosphorylated at Ser364 by Chk2 kinase in response to DNA damage, stabilized, mobilized to nucleus and activated as a transcription factor. It induces apoptosis by activating transcription of the p53 homolog, p73. E2F1 protein consists of 437 amino acids with a molecular mass of 46 kDa.

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy