c-MYC antibody (pSer62)

Catalog No. ABIN3201011

Product Details anti-c-MYC Antibody

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Binding Specificity: pSer62

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity (Details): Expected to react with mouse, rat from the sequence identity.

Purification: Purified

Immunogen: Synthetic peptide containing pSer62 of cMyc

Clone: 33A12E10

Isotype: IgG2b kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-c-MYC antibody (ABIN3201011)

Khanna, Böckelman, Hemmes, Junttila, Wiksten, Lundin, Junnila, Murphy, Evan, Haglund, Westermarck, Ristimäki: "MYC-dependent regulation and prognostic role of CIP2A in gastric cancer." in: Journal of the National Cancer Institute, Vol. 101, Issue 11, pp. 793-805, (2009) (PubMed).

Junttila, Puustinen, Niemelä, Ahola, Arnold, Böttzauw, Ala-aho, Nielsen, Ivaska, Taya, Lu, Lin, Chan, Wang, Grènman, Kast, Kallunki, Sears, Kähäri, Westermarck: "CIP2A inhibits PP2A in human malignancies." in: Cell, Vol. 130, Issue 1, pp. 51-62, (2007) (PubMed).

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc (MYC Products)

Background: CMyc is a proto-oncogene, which is overexpressed in a wide range of human cancers. Myc gene encodes a transcription factor that regulates a great number of genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferase. It can also act as a transcriptional repressor. It regulates cell growth, apoptosis, differentiation and stem cell self-renewal. Previous studies on the phosphorylation of c-Myc have suggested functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK), cell proliferation and cell cycle regulation. Phosphorylation at Ser62 is required for Ras-induced stabilization and is prerequisite for phosphorylation at Thr58 for its degradation.

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect