S100A3 antibody (AA 1-101)

Catalog No. ABIN3201662

Product Details anti-S100A3 Antibody

Target: S100A3 (S100 Calcium Binding Protein A3 (S100A3))

Binding Specificity: AA 1-101

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to S100 Calcium Binding Protein A3 (S100A3)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- MARPLEQAVA AIVCTFQEYA GRCGDKYKLC QAELKELLQK ELATWTPTEF RECDYNKFMS VLDTNKDCEV DFVEYVRSLA CLCLYCHEYF KDCPSEPPCS Q

Specificity: The antibody is a mouse monoclonal antibody raised against S100A3. It has been selected for its ability to recognize S100A3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant S100 Calcium Binding Protein A3 (S100A3) corresdonding to Met1~Gln101 (Accession # P33764) with N-terminal His Tag

Clone: C4

Isotype: IgG1 kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for S100A3

Target: S100A3 (S100 Calcium Binding Protein A3 (S100A3))

Alternative Name: S100 Calcium Binding Protein A3 (S100A3 Products)

Synonyms: S100E antibody, S100 calcium binding protein A3 antibody, S100A3 antibody, S100a3 antibody

Background: S100E, S100-A3

Pathways: S100 Proteins