CD11b antibody (FITC)

Catalog No. ABIN320306

The Mouse Monoclonal anti-CD11b antibody has been validated for FACS. It is suitable to detect CD11b in samples from Rat.

Quick Overview for CD11b antibody (FITC) (ABIN320306)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: OX-42

Product Details anti-CD11b Antibody (FITC)

Purification: Affinity Chromatography on Protein G.

Immunogen: Rat resident peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO/U myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of ABIN182004 for use in functional studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Phosphate Buffered Saline, 1 % Bovine Serum Albumin, Sodium Azide 0.09 %

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gene ID: 10116

UniProt: Q63001

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation