Pepsin antibody

Catalog No. ABIN3208206

Product Details anti-Pepsin Antibody

Target: Pepsin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pepsin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against PP. It has been selected for its ability to recognize PP in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: Native Protein Pepsin

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 0.5-2 μg/mL
• Immunocytochemistry in formalin fixed cells: 5-20 μg/mL
• Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL
• Immunohistochemistry in paraffin section: 5-20 μg/mL
• Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL
• Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 4 °C for frequent use. Stored at -20 °C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Pepsin antibody (ABIN3208206)

Chen, Bao, Zhou, Yao, Chen: "Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord Leukoplakia." in: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, Vol. 164, Issue 1, pp. 160-165, (2021) (PubMed).

Lee, Kwon, Shin, Eun, Shin, Lee: "Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux." in: Sensors (Basel, Switzerland), Vol. 20, Issue 1, (2020) (PubMed).

Gong, Wang, Yang, Sun, Du, Zhang: "Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakia." in: Journal of voice : official journal of the Voice Foundation, Vol. 32, Issue 3, pp. 352-355, (2018) (PubMed).

Target Details for Pepsin

Target: Pepsin

Alternative Name: Pepsin (PP) (Pepsin Products)