PRIM1 antibody (AA 24-73)

Catalog No. ABIN321005

The Rabbit Polyclonal anti-PRIM1 antibody has been validated for WB. It is suitable to detect PRIM1 in samples from Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio) and Bat.

Quick Overview for PRIM1 antibody (AA 24-73) (ABIN321005)

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRIM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRIM1 Antibody

Binding Specificity: AA 24-73

Specificity: Human PRIM1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Guinea pig (100%) Dog (92%) Xenopus (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa24-73 of human PRIM1 (P49642, NP_000937). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Marmoset, Hamster, Dog (92%), Lizard (90%), Xenopus (85%), Beetle (81%), Sea squirt (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PRIM1

Target: PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))

Alternative Name: PRIM1

Background: Name/Gene ID: PRIM1

Synonyms: PRIM1, DNA primase subunit 48, DNA primase 1, Dna primase 49 kd subunit, DNA primase 49 kDa subunit, p49, Primase polypeptide 1, 49  kDa, DNA primase small subunit, Primase p49 subunit

Gene ID: 5557

NCBI Accession: NP_000937

UniProt: P49642

Pathways: Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, SARS-CoV-2 Protein Interactome