GOT1 antibody (N-Term)

Catalog No. ABIN321401

The Rabbit Polyclonal anti-GOT1 antibody is suitable to detect GOT1 in samples from Human, Mouse, Rat, Cow, Pig, Zebrafish (Danio rerio), Monkey, Goat, Guinea Pig, Horse, Rabbit and Bat. It has been validated for WB.

Quick Overview for GOT1 antibody (N-Term) (ABIN321401)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Human, Mouse, Rat, Cow, Pig, Zebrafish (Danio rerio), Monkey, Goat, Guinea Pig, Horse, Rabbit, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GOT1 Antibody

Binding Specificity: N-Term

Specificity: Human GOT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Goat, Rabbit, Horse (100%) Xenopus (92%) Zebrafish (84%).

Purification: Protein A purified

Immunogen: Synthetic peptide from N-Terminus of human GOT1 (P17174, NP_002070). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%), Hamster, Chicken, Xenopus (92%), Rice (85%), Salmon, Zebrafish, Drosophila (84%), Slime mold (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: Aspartate Aminotransferase

Background: Name/Gene ID: GOT1

Synonyms: GOT1, Aspartate Aminotransferase, ASTQTL1, Growth-inhibiting protein 18, Transaminase A, GIG18

Gene ID: 2805

NCBI Accession: NP_002070

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process