IL-1 beta antibody

Catalog No. ABIN327293

The Rat Monoclonal anti-IL-1 beta antibody has been validated for WB and ELISA. It is suitable to detect IL-1 beta in samples from E. coli.

Quick Overview for IL-1 beta antibody (ABIN327293)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: E. coli

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL-1 beta Antibody

Specificity: Recognizes equine IL-1beta. Species sequence homology: 64 % - 72 % aa sequence identity with bovine, feline, goat, human, mouse, ovine, porcine, rabbit, and ratIL-1beta. Does not cross-react with rcaIL-1beta, rcrIL-1beta, rfeIL-1beta, rhIL-1beta, rpIL-1beta, rrIL-1beta rhIL-1H1 or rmIL-17Rbeta.

Purification: Protein G purified

Immunogen: E. coli-derived recombinant equine reqIL-1 beta.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western blot and direct ELISA. Western blot: 1-2 μg/mL. The detection limit is 5 ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is 2 ng/well.

Comment:

Target Species of Antibody: E. coli

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, PBS to a concentration of 0.5 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitute, PBS to a concentration of 0.5 mg/mL.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B

Background: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome