PKC alpha antibody
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- Target See all PKC alpha (PKCa) Antibodies
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Fish
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PKC alpha antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
- Specificity
- Detects an ~80kD protein, corresponding to the apparent molecular mass of PKC alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, fish (rainbow trout), guinea pig, monkey, pig, rabbit and sheep origins. The epitope of this antibody is within the variable region V3 of PKC alpha (the site of limited proteolysis on the native enzyme). This antibody is specific for PKC alpha and does not detect the other PKC isoforms.
- Purification
- Protein G purified
- Immunogen
- Bovine brain PKCa.
- Clone
- MC5
- Isotype
- IgG2a
- Top Product
- Discover our top product PKCa Primary Antibody
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- Application Notes
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Approved: Flo (10 μg/mL), IHC (1:50), IP (12.5 μg/mL), WB (1 μg/mL)
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Bovine
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS 0.1 mM PMSF, 50 % glycerol
- Preservative
- PMSF
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
- Alternative Name
- PRKCA / PKC-alpha (PKCa Products)
- Synonyms
- pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody
- Background
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Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase
Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha - Gene ID
- 5578
- Pathways
- WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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