S100A8 antibody (FITC)
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- Target See all S100A8 Antibodies
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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Reactivity
- Human, Rat, Cow, Pig, Rabbit, Monkey, Cat, Dog, Horse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This S100A8 antibody is conjugated to FITC
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Recognizes human L1 Protein or Calprotectin Molecule. Variable results have been reported for the staining of brain macrophages and microglia. Species cross-reactivity: equine, Cynomolgus monkey, Rhesus monkey, monkey, bovine, baboon, rabbit, canine, feline, porcine, guinea pig and rat.
- Purification
- Protein G purified
- Immunogen
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Human monocytes.
Type of Immunogen: Cells - Isotype
- IgG1
- Top Product
- Discover our top product S100A8 Primary Antibody
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- Application Notes
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Approved: Flo, IHC, IHC-P (1:50)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Flow Cytometry: Neat. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Membrane permeabilization is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 1 % BSA, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
- Alternative Name
- S100A8 / MRP8 (S100A8 Products)
- Synonyms
- 60B8AG antibody, CAGA antibody, CFAG antibody, CGLA antibody, CP-10 antibody, L1Ag antibody, MA387 antibody, MIF antibody, MRP8 antibody, NIF antibody, P8 antibody, Mrp8 antibody, S100A8 antibody, 60B8Ag antibody, AI323541 antibody, B8Ag antibody, CFAg antibody, Caga antibody, p8 antibody, MRP-8 antibody, S100 calcium binding protein A8 antibody, S100 calcium binding protein A8 (calgranulin A) antibody, S100A8 antibody, S100a8 antibody
- Background
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Name/Gene ID: S100A8
Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8 - Gene ID
- 6279
- UniProt
- P05109
- Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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