S100A8 antibody

Catalog No. ABIN6940546

This Mouse Monoclonal antibody specifically detects S100A8 in IHC, IF and FACS. It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey, Guinea Pig, Horse, Baboon and Goat.

Quick Overview for S100A8 antibody (ABIN6940546)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey, Guinea Pig, Horse, Baboon, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MAC387

Product Details anti-S100A8 Antibody

Purpose: Calprotectin / MRP14 / S100A9 / Calgranulin B Antibody

Specificity: Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Affinity Purified monocyte membrane preparation

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Tonsil, lymph node or spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8

Background:

60B8AG, Calgranulin A (CAGA), Calgranulin B (CAGB), Calprotectin L1L subunit, Chemotactic cytokine CP-10, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, L1Ag, Macrophage L1 protein Calprotectin, Migration inhibitory factor related protein 8, Myeloid-related protein 8 (MRP-8 or P8), Myeloid-related protein 14 (MRP-14 or P14), Neutrophil cytosolic 7 kDa protein, NIF, S100A8/S100A9 complex, MA387, Pro-inflammatory S100 cytokine, Urinary stone protein band A

lymph node or spleen., Tonsil

Molecular Weight: MAC387

Gene ID: 6279, 6280, 416073

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins