beta-2 Microglobulin antibody

Catalog No. ABIN334257

The Mouse Monoclonal anti-beta-2 Microglobulin antibody (Clone 2213) (ABIN334257) specifically detects beta-2 Microglobulin in IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for beta-2 Microglobulin antibody (ABIN334257)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2213

Product Details anti-beta-2 Microglobulin Antibody

Specificity: Recognizes native human beta 2 microglobulin, a 119 amino acid component of MHC class I molecules present on all nucleated cells, containing a single domain, Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Also known as B2M, it has been suggested that this protein can be used as a prognostic marker for HIV infection.

Purification: Protein A purified

Immunogen: Highly pure beta-2-microglobulin from human urine.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 8.0, 0.05 % sodium azide, 10 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M / Beta 2 Microglobulin

Background: Name/Gene ID: B2M

Synonyms: B2M, Beta 2 Microglobulin, Beta-2-microglobulin, Beta-2-microglobin

Gene ID: 567

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process