S100A8 antibody

Catalog No. ABIN334264

This Mouse Monoclonal antibody specifically detects S100A8 in IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Pig, Dog, Cat, Monkey and Guinea Pig.

Quick Overview for S100A8 antibody (ABIN334264)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human, Mouse, Rat, Pig, Dog, Cat, Monkey, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MAC 387

Product Details anti-S100A8 Antibody

Specificity: Recognizes neutrophils, monocytes, macrophages and squamous mucosal epithelia. Has been shown as an important marker for identifying macrophages in tissue sections.

Purification: Protein G purified

Immunogen: Affinity purified monocyte membrane preparation.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Tonsil, lymph node or spleen. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4. No preservatives added. No stabilizing proteins added.

Preservative: Azide free

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / MRP8

Background: Name/Gene ID: S100A8

Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8

Gene ID: 6279

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins