S100A8 antibody (AA 2-89)

Catalog No. ABIN3042481

Product Details anti-S100A8 Antibody

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Binding Specificity: AA 2-89

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Protein S100-A8(S100A8) detection. Tested with WB, IHC-P in Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein S100-A8(S100A8) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: S100 calcium binding protein A8
Protein Name: Protein S100-A8

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 (S100A8 Products)

Synonyms: 60B8AG antibody, CAGA antibody, CFAG antibody, CGLA antibody, CP-10 antibody, L1Ag antibody, MA387 antibody, MIF antibody, MRP8 antibody, NIF antibody, P8 antibody, Mrp8 antibody, S100A8 antibody, 60B8Ag antibody, AI323541 antibody, B8Ag antibody, CFAg antibody, Caga antibody, p8 antibody, MRP-8 antibody, S100 calcium binding protein A8 antibody, S100 calcium binding protein A8 (calgranulin A) antibody, S100A8 antibody, S100a8 antibody

Background: S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Synonyms: 60B8Ag antibody|AI323541 antibody|B8Ag antibody|BEE11 antibody|CAGA antibody|Calgranulin-A antibody|Calprotectin L1L subunit antibody| Calprotectin, included antibody|CFAG antibody|CGLA antibody|Chemotactic cytokine CP-10 antibody|CP-10 antibody|Cystic fibrosis antigen antibody|L1Ag antibody|Leukocyte L1 complex light chain antibody|MA387 antibody|MIF antibody|Migration inhibitory factor-related protein 8 antibody|MRP-8 antibody|Myeloid-related protein 8 antibody|Neutrophil cytosolic 7 kDa protein antibody|NIF antibody|p8 antibody|Pro-inflammatory S100 cytokine antibody|Protein S100-A8 antibody|S100 calcium binding protein A8 (calgranulin A) antibody| S100 calcium binding protein A8 antibody|S100 calcium-binding protein A8 antibody|S100A8 antibody|S100A8/S100A9 complex, included antibody|S10A8_HUMAN antibody|Urinary stone protein band A antibody

Gene ID: 20201

UniProt: P27005

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins