ARF1 antibody (C-Term)

Catalog No. ABIN334352

The Goat Polyclonal anti-ARF1 antibody has been validated for WB, ELISA and FACS. It is suitable to detect ARF1 in samples from Human, Rat, Mouse and Dog.

Quick Overview for ARF1 antibody (C-Term) (ABIN334352)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-ARF1 Antibody

Binding Specificity: C-Term

Purpose: ARF1 (variants 1-4)

Sequence: EGLDWLSNQL RNQK

Specificity: This antibody is expected to recognize all four reported isoforms.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EGLDWLSNQLRNQK, from the C Terminus of the protein sequence according to NP_001019397.1, NP_001649.1, NP_001019398.1, NP_001019399.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 18 kDa band observed in lysates of cell lines A431, HeLa, MCF7, NIH3T3, KNRK and MDCK (calculated MW of 20.7 kDa according to Human NP_001649.1, Mouse NP_031502.1 and Rat NP_071963.1). Recommended concentration: 0.3-1 μg/mL. Primary inc

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background: ARF1, ARF 1, ADP-ribosylation factor 1, ADP ribosylation factor 1

Gene ID: 375, 376, 377

NCBI Accession: NP_001019397, NP_001649, NP_001019398, NP_001019399

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process