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ERCC1 antibody (N-Term)

Verified ERCC1 Reactivity: Human WB, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN334392
  • Target See all ERCC1 Antibodies
    ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))
    Binding Specificity
    • 15
    • 11
    • 10
    • 6
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 80
    • 38
    • 24
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 55
    • 24
    • 1
    Goat
    Clonality
    • 55
    • 25
    Polyclonal
    Conjugate
    • 52
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ERCC1 antibody is un-conjugated
    Application
    • 72
    • 43
    • 16
    • 13
    • 13
    • 11
    • 10
    • 8
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Purpose
    ERCC1
    Sequence
    DPGKDKEGVP QPS
    Specificity
    This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence DPGKDKEGVPQPS-C, from the N Terminus of the protein sequence according to NP_973730.1, NP_001974.1.
    Isotype
    IgG
    Top Product
    Discover our top product ERCC1 Primary Antibody
  • Application Notes

    Western Blot: Approx 38 kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6 kDa according to NP_973730.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Comment

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))
    Alternative Name
    ERCC1 (ERCC1 Products)
    Synonyms
    COFS4 antibody, RAD10 antibody, UV20 antibody, Ercc-1 antibody, ercc1 antibody, T12H1.18 antibody, T12H1_18 antibody, UV REPAIR DEFICIENT 7 antibody, UVR7 antibody, ERCC excision repair 1, endonuclease non-catalytic subunit antibody, excision repair cross-complementing rodent repair deficiency, complementation group 1 antibody, DNA excision repair protein ERCC-1 antibody, nucleotide repair protein antibody, excision repair cross-complementation group 1 L homeolog antibody, excision repair cross-complementation group 1 antibody, ERCC1 antibody, Ercc1 antibody, CC1G_04546 antibody, CpipJ_CPIJ013903 antibody, PITG_13719 antibody, ercc1 antibody, ercc1.L antibody
    Background
    ERCC1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), COFS4, UV20, excision repair cross-complementing 1, excision repair protein
    Gene ID
    2067
    NCBI Accession
    NP_973730, NP_001974
    Pathways
    DNA Damage Repair, Production of Molecular Mediator of Immune Response
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