APEX1 antibody

Catalog No. ABIN6254847

Cited in 4+ publications. The Rabbit Polyclonal anti-APEX1 antibody (ABIN6254847) specifically detects APEX1 in WB. The antibody is reactive with Plumbago zeylanica, Alfalfa, Arabidopsis thaliana, Triticum aestivum, Tomato, Spinach, Silene vulgaris, Schima superba, Red pepper, Rape, Proso millet (Panicum miliaceum), Armeria maritima, Oryza sativa, Nicotiana tabacum, Manihot esculenta, Lupine, Liquidambar formosana, Lathyrus sativus, Iris pumila, Echinochloa crus-galli, Dionaea muscipula, Digitaria sanguinalis and Citrus spp. samples.

Quick Overview for APEX1 antibody (ABIN6254847)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Plumbago zeylanica, Alfalfa, Arabidopsis thaliana, Triticum aestivum, Tomato, Spinach, Silene vulgaris, Schima superba, Red pepper, Rape, Proso millet (Panicum miliaceum), Armeria maritima, Oryza sativa, Nicotiana tabacum, Manihot esculenta, Lupine, Liquidambar formosana, Lathyrus sativus, Iris pumila, Echinochloa crus-galli, Dionaea muscipula, Digitaria sanguinalis, Citrus spp.

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APEX1 Antibody

Purpose: Anti-APX | L-ascorbate peroxidase

Cross-Reactivity (Details): Arabidopsis thaliana, Armeria maritima, Brassica napus, Capsicum annuum, Citrus sp., Digitaria sanguinalis, Dionaea muscipula, Echinochla crus-galli, Iris pumila, Lathyrus sativus, Liquidambar formosana, Lupin sp., Manihot esculenta, Medicago sativa, Nicotiana tabacum thylakoid-bound APX, stromal APX, Oryza sativa, Panicum milaceum, Plumbago zeylanica, Schima superba, Silene vulgaris, Solanum lycopersicum, Spinacia oleracea (stromal APX, thylakoid-bound), Triticum aestivum
Not reactive in: Algae, Helianthus annus, Marchantia polymorpha,

Predicted Reactivity: Brassica rapa subsp. oleifera Stromal APX, Glycine max,Glycine soja L-ascorbate peroxidase T,chloroplastic, Medicago truncatula thylakoid-bound APX, Mesembryanthemum crystallinum,Pisum sativum Chloroplast stromal ascorbate peroxidase 12, Solanum lycopersicum thylakoid-bound APX, Spinacia oleracea stromal APX, Theobroma cacao L-APX T isoform 3, Vitis vinifera

Purification: Serum

Immunogen: BSA-conjugated synthetic peptide derived from Arabidopsis thaliana tAPX (thylakoidal ascorbate peroxidase) UniProt: Q42593-1

Application Details

Application Notes: 1 : 2000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-APEX1 antibody (ABIN6254847)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, (2010) (PubMed).

Hideg, Kuos, Schreiber: "Imaging of NPQ and ROS formation in tobacco leaves: heat inactivation of the water-water cycle prevents down-regulation of PSII." in: Plant & cell physiology, Vol. 49, Issue 12, pp. 1879-86, (2008) (PubMed).

Kangasjaervi, Lepistoe, Haennikaeinen, Piippo, Luomala, Aro, Rintamaeki: "Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses." in: The Biochemical journal, Vol. 412, Issue 2, pp. 275-85, (2008) (PubMed).

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APX

Background: APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).

Molecular Weight: 25-38 kDa for A. thaliana

Gene ID: 844085

UniProt: Q42593, Q42592, Q05431, Q1PER6, Q42564

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus