Notch1 antibody (Internal Region)

Catalog No. ABIN335153

The Goat Polyclonal anti-Notch1 antibody is suitable to detect Notch1 in samples from Zebrafish (Danio rerio). It has been validated for ELISA.

Quick Overview for Notch1 antibody (Internal Region) (ABIN335153)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-Notch1 Antibody

Binding Specificity: Internal Region

Purpose: Notch1b (zebrafish)

Sequence: HLGIGNNQDL SHIQ

Cross-Reactivity: Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HLGIGNNQDLSHIQ, from the internal region of the protein sequence according to NP_571377.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Not yet tested. At this stage we are dependent on researchers in the field for further characterization of this product. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: notch1b

Background: Notch1b, notch homolog 1b , fc55e11, wu:fc55e11, etID309871.5, notch receptor protein 1b

NCBI Accession: NP_571377

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development